ProductCategory: Pre-maderecombinantAAV;AAV-Synapsin-iCre;AAV-Syn-iCre;AAV6-Synapsin-iCre;AAV6-Syn-iCre
Description:
CrerecombinaseisatypeItopoisomerasefrombacteriophageP1thatcatalyzesthesite-specificrecombinationofDNAbetweenloxPsites. Crerecombinaseisusedasatooltogeneticallymodifygenes,suchastodeleteasegmentofDNAflankedbyLoxPsitesincellsorexperimentalanimals. ByoptimizingthemammaliancodonusageforCrerecombinase,Creexpressionisimprovedinthemammaliancells.Thiscodon-improvedCre(knownasiCre)transgeneshowslowerCpGcontentfromtheprokaryoticcodingsequence,thusreducingthechancesofepigeneticsilencinginmammals.
AAV6-Syn-iCreisapre-packagedrAAVinSEROtype6(withcapsidfromAAVserotype6and2xITRfromAAVserotype2)whichover-expressesiCrerecombinaseunderhumansynapsinpromoterforexclusiveneuronalexpression.ThisproductusedintheCre-loxsystemasagenetictooltogeneratesite-specificrecombinationofDNAbetweenloxPsitesinculturedcellsandanimalexperiments. Readytouseformat.
Titer: >1x1013 VG/mL
Size: > 30 µL(StdPack)
StorageBuffer: PBS
InfectionProtocol: 
VectorMap: 
